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Substrate solution is then added and color develops in proportion to the amount of Human GSTO2
Then a biotinylated detection antibody specific for Rat IGFBP-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
AFF2 present in the sample is added and binds to antibodies coated on the wells
QRFP present in the sample is added and binds to antibodies coated on the wells
The concentration of Pig BMP2 in the samples is then determined by comparing the OD of the samples to the standard curve
Human TNFSF9 (Tumor Necrosis Factor Ligand Superfamily Member 9) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (TNFSF9) Tumor Necrosis Factor Ligand Superfamily Member 9 ELISA Kit measures Tumor Necrosis Factor Ligand Superfamily Member 9 in samples. The plate has been pre coated with Human TNFSF9 antibody. TNFSF9 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TNFSF9 Antibody is added and binds to TNFSF9 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TNFSF9
