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You can calculate the concentration of Mouse IL-4 in the samples by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Mouse IL-22 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL1h
coli expression system and the target gene encoding Ala26-Arg221 is expressed with a 6His tag at the N-terminus
A standard curve is prepared relating color intensity to the concentration of 17-OHP
Rat ANP (Atrial Natriuretic Peptide) ELISA Kit TIM1 You can calculate the concentrationThis Rat ANP (Atrial Natriuretic Peptide) ELISA Kit uses the competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Rat ANP. During the reaction, Rat ANP in the sample or standard competes with a fixed amount of Rat ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase
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