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Substrate solution is then added and color develops in proportion to the amount of Human H2AC20
The microplate provided in this kit has been precoated with anti ALDH5A1 antibody
The plate has been pre-coated with Mouse HMGB1 antibody
GUCY1A2 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated NASP antibody
Rat H3cit-NE (neutrophil extracellular trap) ELISA Kit APT1 Substrate solution is then addedThe Rat H3cit NE (neutrophil extracellular trap) ELISA Kit measures H3cit NE in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti H3cit NE antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add
