Mouse F8 (Coagulation Factor 8) ELISA Kit Packaging:96 wells The microtiter plate provided in

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DEFb126

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TLR1

The concentration of Rat CALB in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for IL-3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Substrate solution is then added and color develops in proportion to the amount of Human IAP

Mouse F8 (Coagulation Factor 8) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Mouse (F8) Coagulation Factor 8 ELISA Kit measures Coagulation Factor 8 in Mouse samples. The plate has been pre coated with Mouse F8 antibody. F8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse F8 Antibody is added and binds to F8 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated F8 antibody. After incubation unbound Streptavidin HRP is washed away during a washing