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After a washing step that removes unbound materials
The plate has been pre-coated with Human EPHA2 antibody
S100PBP present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Human BLC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Rat SERPING1 Antibody is added and binds to SERPING1 in the sample
Mouse SIRT2 (Sirtuin 2) ELISA Kit Packaging:96 wells After a washing step thatThe Mouse SIRT2 (Sirtuin 2) ELISA Kit measures SIRT2 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti SIRT2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
