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a horseradish peroxidase conjugated mouse anti-Mouse TFPI2 Antibody is added to the wells and binds to TFPI2 in the sample
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CD27BP
Substrate solution is then added and color develops in proportion to the amount of Human ACTN-1
NPPB present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated SELE antibody
Human YWHAB (14-3-3 Protein Beta, Alpha) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouseThe Human (YWHAB) 14 3 3 Protein Beta, Alpha ELISA Kit measures 14 3 3 Protein Beta Alpha in samples. The plate has been pre coated with Human YWHAB antibody. YWHAB present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human YWHAB Antibody is added and binds to YWHAB in the sample. Then Streptavidin HRP is added and binds to the Biotinylated YWHAB antibody. After incubation unbound Streptavidin HRP is washed
