Human ANXA5 (Annexin A5) ELISA Kit Diphtheria Toxin Receptor Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human SEMA3F

You can calculate the concentration of Human PCâ…¢ in the samples by comparing the OD of the samples to the standard curve

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL6

And then biotinylated Mouse ANXA7 Antibody is added and binds to ANXA7 in the sample

Then a biotinylated detection antibody specific for Rat PTGS2/COX-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human ANXA5 (Annexin A5) ELISA Kit Diphtheria Toxin Receptor Substrate solution is then addedThe Human ANXA5 (Annexin A5) ELISA Kit measures ANXA5 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ANXA5 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.