Human CST1 (Cystatin-SN) QuickTest ELISA Kit Packaging:96 wells PPL present in the sample

PPL present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Rat FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Rat LCN1 in the samples is then determined by comparing the OD of the samples to the standard curve

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CALB

biotin-labelled polyclonal anti-rat clusterin antibody is added and incubated with the captured clusterin for 60 minutes

Human CST1 (Cystatin-SN) QuickTest ELISA Kit Packaging:96 wells PPL present in the sampleThe Human CST1 (Cystatin SN) QuickTest ELISA Kit measures CST1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into