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only those wells that contain Human NPTX2
Then a biotinylated detection antibody specific for Human IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human ICAM1 antibody
The concentration of Human TPO in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human PPARd
Mouse SEMA3D (Semaphorin-3D) ELISA Kit Packaging:96 wells only those wells that containThe Mouse (SEMA3D) Semaphorin 3D ELISA Kit measures Semaphorin 3D in Mouse samples. The plate has been pre coated with Mouse SEMA3D antibody. SEMA3D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse SEMA3D Antibody is added and binds to SEMA3D in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SEMA3D antibody. After incubation unbound Streptavidin HRP is washed away during
