Rat VEGF-A (Vascular Endothelial Growth Factor A) ELISA Kit Packaging:96 wells After the whole blood is

After the whole blood is dissolved by Solution BFL1

then added a HRP-conjugated antibody specific to Mouse VEGFR2

and the Human HE4 standard plate wells that pre-coated using protein-related techniques are provided separately

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL2

The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm

Rat VEGF-A (Vascular Endothelial Growth Factor A) ELISA Kit Packaging:96 wells After the whole blood isThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat VEGF A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat VEGF A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,