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Then Streptavidin-HRP is added and binds to the Biotinylated SYT6 antibody
Then a biotinylated detection antibody specific for Mouse IL-1 alpha and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The Human IL-34 ELISA kit is a sandwich enzyme immunoassay
Calculate the concentration of DKK-1 in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD19
Rat AREG (Amphiregulin) QuickTest ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Rat AREG (Amphiregulin) QuickTest ELISA Kit measures AREG in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into individual
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