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And then biotinylated Mouse KEAP1 Antibody is added and binds to KEAP1 in the sample
Substrate solution is then added and color develops in proportion to the amount of Human FAM3D
Then a biotinylated detection antibody specific for Mouse TSLP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human MUC2 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated NUCB2 antibody
Human EGFLAM (Pikachurin) ELISA Kit CD62L And then biotinylated Mouse KEAP1The Human EGFLAM (Pikachurin) ELISA Kit measures EGFLAM in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti EGFLAM antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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