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Substrate solution is then added and color develops in proportion to the amount of Bovine Cystatin-3
Then Streptavidin-HRP is added and binds to the Biotinylated LAX1 antibody
You can calculate the concentration of Human LBP in the samples by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated HSF2 antibody
VWF present in the sample is added and binds to antibodies coated on the wells
Human CD276 (CD276 antigen) QuickTest ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human CD276 (CD276 antigen) QuickTest ELISA Kit measures CD276 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
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