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And then biotinylated Human HTR7 Antibody is added and binds to HTR7 in the sample
The concentration of Rat NRGN in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human AANAT Antibody is added and binds to AANAT in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated FZD5 antibody
a horseradish peroxidase conjugated mouse anti-Human TrkA Antibody is added to the wells and binds to TrkA in the sample
Rat LEP (Leptin) ELISA Kit ANF And then biotinylated Human HTR7The Rat (LEP) Leptin ELISA Kit measures Leptin in Rat samples. The plate has been pre coated with Rat LEPTIN antibody. LEPTIN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LEPTIN Antibody is added and binds to LEPTIN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPTIN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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