FN (Fibronectin) ELISA Kit D2S1473 Rat T in samples (or

Rat T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T

a horseradish peroxidase conjugated mouse anti-Human Cystatin SN Antibody is added to the wells and binds to Cystatin SN in the sample

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit HGF

The concentration of Human IL5 in the samples is then determined by comparing the OD of the samples to the standard curve

which can be directly detected by electrophoresis after the reaction is completed

FN (Fibronectin) ELISA Kit D2S1473 Rat T in samples (orThis FN (Fibronectin) ELISA Kit uses the sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to FN . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are