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And then biotinylated Human POMC Antibody is added and binds to POMC in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated KCNIP4 antibody
Then a biotinylated detection antibody specific for Human Gamma/CXCL9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Unbound cortisol and cortisol enzyme conjugate is washed off by washing buffer
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Bcl-2
Mouse EPO (Erythropoietin) QuickTest ELISA Kit Packaging:96 wells And then biotinylated Human POMCThe Mouse EPO (Erythropoietin) QuickTest ELISA Kit measures EPO in Mouse Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
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