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Then a biotinylated detection antibody specific for Human LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Mouse KRT14
When the sample containing Progranulin is added to the plate
EDNRA present in the sample is added and binds to antibodies coated on the wells
only those wells that contain Rat PDGFBB
Rat RLN3 (Relaxin-3) ELISA Kit INP10 Then a biotinylated detection antibodyThe Rat (RLN3) Relaxin 3 ELISA Kit measures Relaxin 3 in Rat samples. The plate has been pre coated with Rat RLN3 antibody. RLN3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat RLN3 Antibody is added and binds to RLN3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RLN3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate