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Substrate solution is then added and color develops in proportion to the amount of Human I-TAC
Then a biotinylated detection antibody specific for Mouse ApoM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
it binds with CHIA bound to precoated antibody
The concentration of MC4R in the sample was calculated by drawing a standard curve
ODAM present in the sample is added and binds to antibodies coated on the wells
Mouse FGF7/KGF (Fibroblast Growth Factor 7) ELISA Kit SF Substrate solution is then addedThe Mouse FGF7 KGF (Fibroblast Growth Factor 7) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse FGF7 KGF. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse FGF7 KGF and Avidin Horseradish Peroxidase (HRP) conjugate are added successively
