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Then a biotinylated detection antibody specific for Mouse PDGF-AA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
And then biotinylated Human PHF7 Antibody is added and binds to PHF7 in the sample
and the color intensity is directly proportional to the amount of Human Ephrin-A1 present
Then Streptavidin-HRP is added and binds to the Biotinylated CPNE2 antibody
The plate has been pre-coated with Human MSTN antibody
Human SRGN (Serglycin) ELISA Kit ANGPTL3 Then a biotinylated detection antibodyThe Human (SRGN) Serglycin ELISA Kit measures Serglycin in samples. The plate has been pre coated with Human SRGN antibody. SRGN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SRGN Antibody is added and binds to SRGN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SRGN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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