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Substrate solution is then added and color develops in proportion to the amount of Human ATG3
the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue colored product that is directly proportional to the amount of leptin present
Unbound streptavidin HRP conjugate is removed by a washing step
then added a HRP-conjugated antibody specific to Human GH
Glucocorticoid receptor Rabbit Polyclonal Antibody
Rat HSL (Hormone Sensitive Lipase) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Rat (HSL) Hormone Sensitive Lipase ELISA Kit measures Hormone Sensitive Lipase in Rat samples. The plate has been pre coated with Rat LIPE antibody. LIPE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LIPE Antibody is added and binds to LIPE in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LIPE antibody. After incubation unbound Streptavidin HRP is washed away during
