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Then a biotinylated detection antibody specific for Mouse MIP-1Beta and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Human CD247
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF6
The OD value is proportional to the concentration of Human IL-18
Then a biotinylated detection antibody specific for Human EMMPRIN/CD147 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human ACY1 (Aminoacylase-1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (ACY1) Aminoacylase 1 ELISA Kit measures Aminoacylase 1 in samples. The plate has been pre coated with Human ACY1 antibody. ACY1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ACY1 Antibody is added and binds to ACY1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ACY1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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