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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig IL-12p40
Then a biotinylated detection antibody specific for Rat SELE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
a horseradish peroxidase conjugated mouse anti-Human carbonic anhydrase XII Antibody is added to the wells and binds to carbonic anhydrase XII in the sample
The concentration of Mouse BNP in the samples is then determined by comparing the OD of the samples to the standard curve
POU domain
MACC1 (Metastasis Associated In Colon Cancer 1) ELISA Kit (Small Sample) Packaging:48 wells Standards or samples are addedThe MACC1 (Metastasis Associated In Colon Cancer 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human MACC1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human MACC1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB