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Substrate solution is then added and color develops in proportion to the amount of Human ABL2
and the color intensity is directly proportional to the amount of Human LAIR2 present
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MYBPC1
This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding
And then biotinylated Mouse CUBN Antibody is added and binds to CUBN in the sample
Human LMOD3 (Leiomodin 3) ELISA Kit IDDM2 Substrate solution is then addedThe Human (LMOD3) Leiomodin 3 ELISA Kit measures Leiomodin 3 in samples. The plate has been pre coated with Human LMOD3 antibody. LMOD3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LMOD3 Antibody is added and binds to LMOD3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LMOD3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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