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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SDF1
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 46 nm
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MYH8
Substrate solution is then added and color develops in proportion to the amount of Mouse GPI
You can calculate the concentration of Human PCT in the samples by comparing the OD of the samples to the standard curve
Human CD83 (CD83 antigen) QuickTest ELISA Kit CD66E Standards or samples are addedThe Human CD83 (CD83 antigen) QuickTest ELISA Kit measures CD83 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples into
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