Rat B-NGF (Beta Nerve Growth Factor) ELISA Kit Packaging:96 wells DHFR present in the sample

DHFR present in the sample is added and binds to antibodies coated on the wells

Then Streptavidin-HRP is added and binds to the Biotinylated VCAM1 antibody

Then Streptavidin-HRP is added and binds to the Biotinylated EFNB1 antibody

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MIP1a

Then a biotinylated detection antibody specific for Human FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat B-NGF (Beta Nerve Growth Factor) ELISA Kit Packaging:96 wells DHFR present in the sampleThe Rat (B NGF) Beta Nerve Growth Factor ELISA Kit measures Beta Nerve Growth Factor in Rat samples. The plate has been pre coated with Rat NGF antibody. NGF present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NGF Antibody is added and binds to NGF in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NGF antibody. After incubation unbound Streptavidin HRP is washed away during a