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Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Then Streptavidin-HRP is added and binds to the Biotinylated CDK5RAP2 antibody
Substrate solution is then added and color develops in proportion to the amount of Rat CALB
The assay plate has been pre-coated with mouse anti-Human BCAM monoclonal antibody
Only those wells that contain Human Flt3L
Rat OCM (Oncomodulin) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat (OCM) Oncomodulin ELISA Kit measures Oncomodulin in Rat samples. The plate has been pre coated with Rat OCM antibody. OCM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat OCM Antibody is added and binds to OCM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OCM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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