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Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm
The concentration of Universal LTD4 in tested samples can be calculated by comparing the OD of the samples to the standard curve
Then a biotinylated detection antibody specific for Human NE/ELA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Only those wells that contain Mouse D-LDH
Ubiquitin (6G6) Mouse mAb Packaging:50 uL Universal 4-HNE in the sampleUbiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome.
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