Human GCA (Grancalcin) ELISA Kit Packaging:96 wells U2 present in the sample

U2 present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human C1INH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The OD value is proportional to the concentration of Mouse GRN

Substrate solution is then added and color develops in proportion to the amount of Human UCN3

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BTLA

Human GCA (Grancalcin) ELISA Kit Packaging:96 wells U2 present in the sampleThe Human GCA (Grancalcin) ELISA Kit measures GCA in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti GCA antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then,