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The concentration of TNFSF4 in the samples is then determined by comparing the OD of the samples to the standard curve
Recombinant Human Complement Factor H-related Protein 4 is produced by our Mammalian expression system and the target gene encoding Glu20-Glu578 is expressed with a 6His tag at the C-terminus
And then biotinylated Human BPI Antibody is added and binds to BPI in the sample
NFkB p65 is also acetylated at K122
The 5HTR5B (5-Hydroxytryptamine Receptor 5B) ELISA Kit is a Sandwich enzyme immunoassay
Human HRG (Histidine Rich Glycoprotein) ELISA Kit Packaging:96 wells The concentration of TNFSF4 inThe Human (HRG) Histidine Rich Glycoprotein ELISA Kit measures Histidine Rich Glycoprotein in samples. The plate has been pre coated with Human HRG antibody. HRG present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human HRG Antibody is added and binds to HRG in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HRG antibody. After incubation unbound Streptavidin HRP is washed away