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Calculate the concentration of PR3-ANCA in the sample by plotting standard curve
Then a biotinylated detection antibody specific for Porcine SPARCL1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The plate has been pre-coated with Human ENPP3 antibody
Then Streptavidin-HRP is added and binds to the Biotinylated SPAM1 antibody
Substrate solution is then added and color develops in proportion to the amount of Human MCM6
Bovine IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA Kit bHLHe78 Calculate the concentration of PR3-ANCAThe Bovine IP 10 CXCL10 (Interferon Gamma Induced Protein 10kDa) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Bovine IP10. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Bovine IP10 and Avidin Horseradish Peroxidase (HRP) conjugate are added
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