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The concentration of Mouse E3 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated LPO antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human N4BP2
Substrate solution is then added and color develops in proportion to the amount of Human MCOLN1
AGER present in the sample is added and binds to antibodies coated on the wells
Mouse M-CSF (Macrophage Colony-Stimulating Factor) QuickTest ELISA Kit Packaging:96 wells The concentration of Mouse E3The Mouse M CSF (Macrophage Colony Stimulating Factor) QuickTest ELISA Kit measures M CSF in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot
