Substrate solution is then added and color develops in proportion to the amount of Human WNT9A
The concentration of Human Igk in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated SPAM1 antibody
The Human (AGGF1) Angiogenic Factor with G Patch and Fha Domains 1 ELISA Kit measures Angiogenic Factor with G Patch and Fha Domains 1 in samples
Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human IDH2 (IsocitRate dehydrogenase [NADP], mitochondrial) ELISA Kit MOT2 Substrate solution is then addedThe Human IDH2 (IsocitRate dehydrogenase [NADP], mitochondrial) ELISA Kit measures IDH2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IDH2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add