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Substrate solution is then added and color develops in proportion to the amount of Human FSTL4
The plate has been pre-coated with Sheep PRL antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TGFb1
coli expression system and the target gene encoding His23-Cys155 is expressed
Then a biotinylated detection antibody specific for SOST and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Human PTH (Parathyroid Hormone) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (PTH) Parathyroid Hormone ELISA Kit measures Parathyroid Hormone in samples. The plate has been pre coated with Human PTH antibody. PTH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTH Antibody is added and binds to PTH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PTH antibody. After incubation unbound Streptavidin HRP is washed away during a washing
