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Substrate solution is then added and color develops in proportion to the amount of Mouse Ddit3
After 30 minutes incubation and the last washing step
Then a biotinylated detection antibody specific for Human CRYAlphaB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Calculate the concentration of ETFDH in the sample by plotting standard curve
The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit CG
Human NNE (Non-Neuronal Enolase) ELISA Kit TNFSF2 Substrate solution is then addedThe Human NNE (Non Neuronal Enolase) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human NNE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NNE and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate
