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And then biotinylated Human MC5R Antibody is added and binds to MC5R in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated DCT antibody
The plate has been pre-coated with Mouse TLR2 antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATXN10
The concentration of Rat TSLP in the samples is then determined by comparing the OD of the samples to the standard curve
Rat ADCY10 (Adenylate cyclase type 10) ELISA Kit Packaging:96 wells And then biotinylated Human MC5RThe Rat ADCY10 (Adenylate cyclase type 10) ELISA Kit measures ADCY10 in Rat Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti ADCY10 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
