Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit Packaging:48 wells The assay makes use of

The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for adiponectin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of adiponectin is conjugated to biotin (biotin conjugate)

Then a biotinylated detection antibody specific for Canine PYGM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30

Substrate solution is then added and color develops in proportion to the amount of Human PSAP

The plate has been pre-coated with Mouse EFNA1 antibody

Rat IGFBP-3 (Insulin-Like Growth Factor Binding Protein 3) ELISA Kit Packaging:48 wells The assay makes use ofThe Rat IGFBP 3 (Insulin Like Growth Factor Binding Protein 3) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Rat IGFBP 3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGFBP 3 and Avidin Horseradish Peroxidase (HRP) conjugate are added