Human CDK-4 (Cyclin-dependent Kinase 4) ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human FABP4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human YBX3

Then Streptavidin-HRP is added and binds to the Biotinylated HSPB1 antibody

The assay plate has been pre-coated with mouse anti-Mouse Fetuin A monoclonal antibody

Then a biotinylated detection antibody specific for Human COMP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human CDK-4 (Cyclin-dependent Kinase 4) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (CDK 4) Cyclin dependent Kinase 4 ELISA Kit measures Cyclin Dependent Kinase 4 in samples. The plate has been pre coated with Human CDK4 antibody. CDK4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CDK4 Antibody is added and binds to CDK4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CDK4 antibody. After incubation unbound Streptavidin HRP is washed away