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Substrate solution is then added and color develops in proportion to the amount of Human GOLGA5
Then a biotinylated detection antibody specific for Mouse GROGamma/CXCL3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse IL4 in the samples is then determined by comparing the OD of the samples to the standard curve
The plate has been pre-coated with Bovine TIMP2 antibody
only those wells that contain Human KL-6
Mouse RPTN (Repetin) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse (RPTN) Repetin ELISA Kit measures Repetin in Mouse samples. The plate has been pre coated with Mouse RPTN antibody. RPTN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse RPTN Antibody is added and binds to RPTN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RPTN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
