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Then Streptavidin-HRP is added and binds to the Biotinylated MEP1B antibody
And then biotinylated Mouse NR4A2 Antibody is added and binds to NR4A2 in the sample
You can calculate the concentration of ACE2 in the samples by comparing the OD of the samples to the standard curve
Substrate solution is then added and color develops in proportion to the amount of Human ACY1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BMP3
Human BMP-2 (Bone Morphogenetic Protein 2) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human BMP 2 (Bone Morphogenetic Protein 2) ELISA Kit measures BMP 2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti BMP 2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated