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Universal 4-HNE in the sample or standard competes with a fixed amount of Universal 4-HNE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal 4-HNE
The OD value is proportional to the concentration of Human GAL9
then added a HRP-conjugated antibody specific to Rat 25-OH-D to each microplate well and incubated
only those wells that contain Mouse INS
You can calculate the concentration of Human ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve
Human BAX (Bcl-2 Associated X Protein) ELISA Kit SCYB11 Universal 4-HNE in the sampleWith about 12 known citations, the Human BAX (Bcl 2 Associated X Protein) ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Human BAX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BAX and Avidin Horseradish Peroxidase (HRP) conjugate are added