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Substrate solution is then added and color develops in proportion to the amount of Human TGM3
The plate has been pre-coated with Human CGN antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse ABAT
Calculate the concentration of LIF in the sample by plotting standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NR1D1
Human IL-18 (Interleukin 18) ELISA Kit TNFRSF6 Substrate solution is then addedThe Human IL 18 (Interleukin 18) ELISA Kit measures IL 18 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection
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