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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MMP-9
also known as lactotransferrin (LTF)
Universal LTD4 in the sample or standard competes with a fixed amount of Universal LTD4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal LTD4
The OD value is proportional to the concentration of Human SAA
Then a biotinylated detection antibody specific for Rat C3a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Flotillin-1 Monoclonal Antibody (6C10) Packaging:50 uL Standards or samples are addedFlotillin 1 Mouse Monoclonal Antibody (6C10). Isotype: IgG1. Species Reactivity: Mouse, Rat. Source: Mouse. Antigen: Synthetic Peptide. Composition and storage: 1 mg mL in PBS, pH 7. 4, containing 0. 02% sodium azide and 50% glycerol. Store at 20C. Stable for one year from the date of shipment.
