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The concentration of Mouse TGFb3 in the samples is then determined by comparing the OD of the samples to the standard curve
And then biotinylated Human A2ML1 Antibody is added and binds to A2ML1 in the sample
SEMA3F present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated DNAH11 antibody
Substrate solution is then added and color develops in proportion to the amount of Human CACYBP
Rat TOP2 (Topoisomerase 2) ELISA Kit Packaging:96 wells The concentration of Mouse TGFb3The Rat (TOP2) Topoisomerase 2 ELISA Kit measures Topoisomerase 2 in Rat samples. The plate has been pre coated with Rat TOP2A antibody. TOP2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat TOP2A Antibody is added and binds to TOP2A in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TOP2A antibody. After incubation unbound Streptavidin HRP is washed away during a washing