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Substrate solution is then added and color develops in proportion to the amount of Mouse SMOC2
The reaction is stopped by adding an acidic stop solution
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human proteasome
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VTN
And then biotinylated Human FGF18 Antibody is added and binds to FGF18 in the sample
Human IRS-4 (Insulin Receptor Substrate 4) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (IRS 4) Insulin Receptor Substrate 4 ELISA Kit measures Insulin Receptor Substrate 4 in samples. The plate has been pre coated with Human IRS4 antibody. IRS4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human IRS4 Antibody is added and binds to IRS4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated IRS4 antibody. After incubation unbound Streptavidin HRP is washed
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