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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CT1
The concentration of Human HGF in the samples is then determined by comparing the OD of the samples to the standard curve
The concentration of Human COL13 in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Rabbit EGF
Then Streptavidin-HRP is added and binds to the Biotinylated CRHBP antibody
Human PAH (Phenylalanine-4-hydroxylase) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Human (PAH) Phenylalanine 4 hydroxylase ELISA Kit measures Phenylalanine 4 hydroxylase in samples. The plate has been pre coated with Human PAH antibody. PAH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PAH Antibody is added and binds to PAH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PAH antibody. After incubation unbound Streptavidin HRP is washed away
