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Substrate solution is then added and color develops in proportion to the amount of Human MAMDC2
You can calculate the concentration of Human PAP in the samples by comparing the OD of the samples to the standard curve
The Human CK-18/KRT18 (Cytokeratin 18) ELISA kit uses the Sandwich-ELISA principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VCAM1
And then biotinylated Human HADH Antibody is added and binds to HADH in the sample
Human SAA4 (Serum Amyloid A-4 Protein) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (SAA4) Serum Amyloid A 4 Protein ELISA Kit measures Serum amyloid A 4 protein in samples. The plate has been pre coated with Human SAA4 antibody. SAA4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SAA4 Antibody is added and binds to SAA4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SAA4 antibody. After incubation unbound Streptavidin HRP is washed away
