Multi-Species Vitamin D3 ELISA Kit AR only those wells that contain

only those wells that contain Human IL-2Ra/CD25 and HRP-conjugated antibody will exhibit a change in color

Then a biotinylated detection antibody specific for Human MUC5AC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

The concentration of Rat TNF-alpha in the samples is then determined by comparing the OD of the samples to the standard curve

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VEGF-A

The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AGER

Multi-Species Vitamin D3 ELISA Kit AR only those wells that containThe Vitamin D3 ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Universal VD3. During the reaction, Universal VD3 in the sample or standard competes with a fixed amount of Universal VD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal VD3. Excess conjugate and unbound sample or standard are washed away, and Avidin Horseradish Peroxidase