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Substrate solution is then added and color develops in proportion to the amount of Human RAMP1
The OD value is proportional to the concentration of Chicken GROAlpha/CXCL1
Then Streptavidin-HRP is added and binds to the Biotinylated C19orf59 antibody
Only those wells that contain Mouse EGF
Calculate the concentration of DNAJB3 in the sample by plotting standard curve
Chicken IFN-Gamma (Interferon Gamma) ELISA Kit MS5 Substrate solution is then addedThe Chicken IFN Gamma (Interferon Gamma) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Chicken IFN Gamma. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Chicken IFN Gamma and Avidin Horseradish Peroxidase (HRP) conjugate are added successively