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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MIg
Substrate solution is then added and color develops in proportion to the amount of Human TRIB3
Then a biotinylated detection antibody specific for Human NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse CYR61 in the samples is then determined by comparing the OD of the samples to the standard curve
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MSP
Chicken ESTROGEN ELISA Kit Packaging:96 wells Standards or samples are addedThe Chicken ESTROGEN ELISA Kit measures Estrogen in Chicken Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Estrogen. Estrogen in the sample or standard competes with a fixed amount of biotin labeled Estrogen for sites on a pre coated antibody specific to Estrogen. Free components are washed off.
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