Human HSD3B1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA Kit Packaging:96 wells And then biotinylated Rat LIF

And then biotinylated Rat LIF Antibody is added and binds to LIF in the sample

the TMB substrate (enzyme substrate) is added which reacts with HRP to form a blue-colored product that is inversely proportional to the amount of pregnenolone present

The concentration of Human FCN1 in the samples is then determined by comparing the OD of the samples to the standard curve

The Sp1 (Specificity Protein 1) ELISA Kit is a Sandwich enzyme immunoassay

Then a biotinylated detection antibody specific for Human HAase and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human HSD3B1 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1) ELISA Kit Packaging:96 wells And then biotinylated Rat LIFThe Human HSD3B1 (3 beta hydroxysteroid dehydrogenase Delta 5 >4 isomerase type 1) ELISA Kit measures HSD3B1 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with HSD3B1. HSD3B1 in the sample or standard competes with a fixed amount of biotin labeled HSD3B1 for sites on a pre coated antibody